KyoungSu ChoiP1, Kyu Tae Park2

1Department of Biology, Kyungpook National University, Daegu, Korea; 2Advanced Bio-resource Research Center, Kyungpook National University, Daegu, Korea

Rubus takesimensisis a Korean endemic species restricted to Ulleung Island and provides a useful system for studying genome evolution within the genus Rubus. Here, we present a high-quality draft genome assembly of R. takesimensis generated using PacBio HiFi long-read sequencing. The final assembly spans 522.5 Mb with a contig N50 of 98.9 kb and a maximum contig length of 36.8 Mb, demonstrating high continuity. The assembly shows high structural consistency with other Rubusgenomes and exceptional base-level accuracy with a Merqury Quality Value (QV) of 50.69. Analysis of k-mer frequency distributions using Jellyfish and GenomeScope indicated substantial sequence duplication associated with abundant repetitive elements. Transposable elements, particularly LTR retrotransposons of the Gypsy and Copia superfamilies, account for a large proportion of the genome.

Genome annotation predicted 62,719 protein-coding genes, and BUSCO analysis recovered 98.4% of conserved orthologs (rosales_odb12), supporting the high completeness of the assembly. Comparative synteny analyses with related Rubus species revealed strong chromosomal collinearity, and Ks distribution analysis identified signatures of both recent duplication events and an ancient whole-genome duplication shared within Rosaceae. These results provide a genomic foundation for future studies of diversification and genome evolution in Rubus.